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The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. The technique has revolutionized immunology and.
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THE OPTIMISATION OF THE ENZYME LINKED IMMUNOSORBANT ASSAY (ELISA) FOR THYROID PEROXIDASE AUTOANTIBODIES (TPO) AUTOANTIBODIES (aAbs) By STUART COOK A thesis submitted to the University of Birmingham for the degree of MASTER OF RESEARCH School of Chemistry College of Engineering the Physical Sciences University of Birmingham October 2009.
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